Re: F4/80 murine mac marker

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From:Santiago =?ISO-8859-1?Q?L=F3pez?= <ivpm26@cc.uab.es>
To:Barbara Daffin Wright <bwright@gene.COM>
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Date:Wed, 07 Apr 1999 18:18:11 +0200
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We use the F4/80 on formalin-fixed and paraffin embedded mouse tissues
using 0.1% protease XIV 8 min at 37C  for antigen retrieval. We have
good inmunostaining of resident and circulating macrophages but not of
those present within inflammatory lesions. Does anyone has the same
problem using this antibody? What could be the reason?
Thanks,

--
Santiago Lˇpez Ortigosa
Dpt. Patologia i Produccions Animals
Facultat VeterinÓria. Universitat Aut˛noma de Barcelona
08193 Bellaterra (Barcelona)
Spain
Tel. 93.581.31.45
Fax 93.581.31.42






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