| From: | Barbara Daffin Wright <bwright@gene.COM> |
| To: | "'histonet'" <histonet@Pathology.swmed.edu>, "Connolly, Brett" <brett_connolly@merck.com> |
| Reply-To: | |
| Date: | Tue, 06 Apr 1999 09:59:51 -0700 |
| Content-Type: | text/plain; charset=us-ascii |
Brett, We successfully stain with F4/80 (Serotec #MCAP497) on paraffin embedded mouse tissues. We use spleen as a positive control. 0.4% pepsin at 37C for 10 minutes for antigen retrieval and an overnight at 4C incubation in a 10ug/ml dilution of F4/80. If you want the procedure just let me know. Thanks Barb Wright