Re: Cleaning of disposable blades
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 07 Apr 1999 09:42:26 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Freida,
The first thing I was taught about disposable blades was that they must be
cleaned before use. Cleaning is not an elaborate procedure, a simple dip
in a 100 ml specimen container of absolute ethanol, & a careful wipe with
an ordinary facial tissue. In the last lab I ran I used Sturkey polymer
coated low profile blades, they come with an instruction to rinse in
ethanol before use.
Why? in every case a cleaned blade will cut thinner, smoother, cleaner
sections than one that has been used without cleaning.
The only exception to this rule is the blades I used in my cryostat, there
I used Sturkey gold coated high profile blades straight from the box. They
cut beautifully from ~3 - 60 um everthing from gels to bone & sections of
entire mice.
Hope this helps.
Regards
Rob W.
At 12:57 PM 4/6/99 -0400, you wrote:
>I am very curious as to how many of you clean your disposable blades before
>use. If you do clean, what do you use?
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.unsw.edu.au/clients/microbiology/CAF.html
(Under development)
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