Hi All,

[  I thought that subject line might grab a few!   ;-)      ]
I have a graduate 
student that has injected rats with hyperimmune-rabbit serum. We want 
to determine if the rabbit-IgG is attaching to the nerves, using  IHC 
in FFPE tissues. 

I tried to stain directly with my Goat anti-rabbit (HRP), just
because I had it on hand; but it isn't working. Too much 
background (surprise, surprise). 

Does anyone else have experience with anything similar?
Should I expect, that any unconjugated anti-rabbit Ab will work?
Is this similar to staining for autantibodies? 
Ag retreival recommendation??
All opinions welcome!                                           
Cheers! Greg
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851