Rat nerves & Rabbit Abs

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From:Greg Dobbin <dobbin@Upei.CA>
Date:Tue, 13 Apr 1999 16:30:20 -0400
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Hi All,

[  I thought that subject line might grab a few!   ;-)      ]
I have a graduate 
student that has injected rats with hyperimmune-rabbit serum. We want 
to determine if the rabbit-IgG is attaching to the nerves, using  IHC 
in FFPE tissues. 

I tried to stain directly with my Goat anti-rabbit (HRP), just
because I had it on hand; but it isn't working. Too much 
background (surprise, surprise). 

Does anyone else have experience with anything similar?
Should I expect, that any unconjugated anti-rabbit Ab will work?
Is this similar to staining for autantibodies? 
Ag retreival recommendation??
All opinions welcome!                                           
Cheers! Greg
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851

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