Dear Barb,

We are doing steam antigen retrieval using a Black and Decker vegetable
steamer for 20 minutes in 10% HIER citrate buffer pH 6.0 on everything
EXCEPT breast tissue.  For that we have gone to using a water bath at 80
degrees for 2 hours, also in HIER, according to the reference below.  It
is the only method we found to be gentle enough for the breast sections.


Elias, Jules M. and Margiotta, Michele:  Low Temperature Antigen
Restoration 
Of Steroid Hormone Receptor Proteins in Routine Paraffin Sections, The 
Journal of Histotechnology, Vol.20, No.2, June,1997.



Diana Goodwin, HT
Trenton, NJ

> ----------
> From: 	Terrett, Barb[SMTP:Barb_Terrett@pmh.toronto.on.ca]
> Sent: 	Thursday, March 25, 1999 12:58 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	steaming slides
> 
> Would anyone who is steaming their slides be willing to send me their
> protocol? Also any references regarding the steaming of slides? Do you
> find
> that you are having fewer problems with tissue falling off
> (particularly
> breast and B5 nodes)?
> Thank you for your time
> b
>