Joanne,

What dilution are you using?  I use the novacastra 1A6 @ 1:80 for 25 min
@ room temp. with very good results.




Diana Goodwin, HT
Trenton, NJ

> ----------
> From: 	Suder, Joanne[SMTP:JC_Suder@fccc.edu]
> Sent: 	Monday, April 05, 1999 1:21 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	Progesterone clone 1A6
> 
> Histonetters,
>   Having a background problem with PR purchased through Novacastra
> clone
> 1A6. Our routine has the slides in Ab overnight at 4o. We have
> recently been
> seeing background staining so we tried it a both rt and 4o. We're
> still
> getting the background staining. Any suggestions?? Please reply
> JC_SUDER@FCCC.EDU
>