RE: Manual or automatic staining

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From:"Ms Louise Taylor" <>
Date:Thu, 15 Apr 1999 08:59:31 GMT + 2.00


Agreed! First know the rules before you break 'em!
louise taylor
South Africa

On 14 Apr 99 at 9:45, wrote:


These are just my own personal thoughts on this subject and do not necessarily reflect those of the company for which I work.
I feel that in the teaching process starting with the basics and being able to master the basics first is best....After that,  move on to differences between manual and automated staining and the  v


Sent:  Tuesday, April 13, 1999 8:09 PM
Subject:  Manual or automatic staining

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Small quick question for my learned colleagues. I am revising the
for a subject I teach called Histotechniques. This subject teaches the
basics of histology and techniques used in processing, microtomy and

My question relates to staining techniques. The theory covers quite
comprehensively the theoretical aspects of staining principles however
during the practical sessions, we ask the students to manual stain using
coplin jars. Each student has their own set of stains and 'experiments'
getting the H&E stain right for sections they have cut.

As workers at the coal face / prospective employers / future teachers
these students abandon their manual staining and revert to an automatic
alternative. Is automatic staining preparing them better for industry,
all I do test their staining 'troubleshooting' in assignments and exams.

Before the howls, I might add that in support of some arguments on this
listserv, I too feel that not enough attention is given to histology at
university level, and that many students 'hate' histology even before they
have completed their degree. I want to add more exciting things to my
(special stains, immunostaining etc .. I'll leave this for later posts).
is it better to move up to automatic staining, thereby using the students
limited time more efficiently to make room for more advanced material.

I would especially like to hear from Australian Histonetters.

Thanks in advance.

Todd L. Walker
Lecturer in Cytology / Histology
School of Biomedical Sciences
Charles Sturt University
PO Box 588
Wagga Wagga

Tel: 61 2 69332541
Fax: 61 2 69332587

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