Shirley,

I attain better cryotomy sectioning of trachea in OCT at  6um when the
cryostat temperature is lowered to between -25 to -30 degree C.

Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center

	----------
	From:  Chu, Shirley [SMTP:schu@megabios.com]
	Sent:  Friday, April 02, 1999 11:59 AM
	To:  HistoNet@pathology.swmed.edu
	Subject:  Cutting Tracheas

	I have a colleague that is sectioning OCT embbeded rat tracheas with
carotid
	arteries attached.  She is having trouble cutting them at around
6-8um in
	thickness.  Are there any recommedations or helpful suggestions as
to how to
	handle sectioning this tissue--temperature, etc?  The tissues were
frozen
	down in isopentane cooled on liquid nitrogen and she is cutting them
on a
	Leica cryostat.
	Shirley Chu
	Valentis Corp
	Burlingame CA