RE: Amplification system

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From:Patsy.Ruegg@UCHSC.edu
To:grazyna.wieczorek@pharma.Novartis.com
Reply-To:
Date:Sat, 03 Apr 1999 18:00:16 -0700
Content-Type:text/plain; charset="windows-1252"

 
Usually the reason you need amplification is because of formalin fixation
masking the antigen site, why would you want to use an amplified detection
for frozen sections?
Patsy Ruegg
-----Original Message-----
From: grazyna.wieczorek@pharma.Novartis.com
To: Histonet@Pathology.swmed.edu
Sent: 3/31/99 6:19 AM
Subject: Amplification system

Dear Users,

I would like to ask you for your opinion and advice. I know that Dako
produces a
Catalyzed Signal Amplification System which supposes to be used with
mouse
monoclonal antibodies and formalin-fixed, paraffin embedded tissues. I
would
like such system (based on biotinyl tyramide) for frozen sections. Has
anyone an
experience with Dako's or other (can be also from other company)
amplification
system on frozen, acetone fixed tissues?

With wishes "Happy Easter",
Grazyna
grazyna.wieczorek@pharma.novartis.com





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