Prolonged fixation
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From: | Barry Rittman <brittman@mail.db.uth.tmc.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 08 Apr 1999 12:49:41 -0500 |
Content-Type: | text/plain; charset=us-ascii |
A while ago, there was a discussion re how long a formalin fixative for
EM could be stored before being considered unsuitable. By chance I came
across an article which addresses this point :
Mount SL., Schwarz JE and Taatjes DJ. Prolonged storage of fixative
for electron microscopy: effects on tissue preservation for diagnostic
specimens.
Ultrastructural Pathology 21: 195-200. 1997. The abstract states:
"Prolonged storage of Karnovsky's fixative, at both 4 and -20 degrees C,
is possible in the electron microscope setting. Ultrastructural detail
was not compromised in specimens processed in fixative that had been
stored for 6 months. Evidence of smooth muscle and neuroendocrine
differentiation was present in the form of actin filaments/dense bodies
and neurosecretory granules, respectively. No difference in preservation
was detected between specimens fixed in freshly prepared Karnovsky's
fixative and fixative that had been stored at either 4 or -20 degrees C
for up to 6 months. Thuis community hospitals can be provided with
Karnovsky's fixative on a semiannual basis for surgical pathology
specimens that may require electron microscopy for diagnosis."
I guess those of us who prefer to make the fixative up fresh are being
somewhat conservative.
Barry
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