PAS and Freida's comments
<< Previous Message | Next Message >>
From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 08 Apr 1999 10:40:24 -0600 |
Content-Type: | |
I think it is also important to know what the pathologists might be looking
for, PAS for fungus, glycogen, basement membrane, mucin, etc indicate to
how I need to do the method. In fact, when they ask for a PAS for fungus,
I use Chromic acid as the oxidizer, not perodic acid.
Let me count the ways to do PAS!! I have more than one PAS protocol for
different things.
I also counterstain with hematoxylin for a shorter time, since the periodic
acid will enhance hematoxylin staining by converting the DNA/RNA COOH groups
to aldehydes, with the chance that you can overwhelm the PAS. In fact, this
is a way to restore hematoxylin staining when tissues have been stored in
NBF too long, and the pH changes. A periodic acid step before hematoxylin
staining often restores the nuclear staining.
Full of sidelights today, must be Springtime!
Gayle Callis
<< Previous Message | Next Message >>