Laser capture microdissection (LCM)

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From:Jamie Erickson <>
Date:Thu, 15 Apr 1999 09:44:15 -0400

Hi all,
    This message is from a guy in our lab about LCM and RNAses in hematoxylin can anyone help?

I will be doing LCM on H&E-stained spleen cryosections and I have a question regarding the staining.  As these captured cells will be used for RNA analysis, do I need to be concerned about using commercially available, pre-made hematoxylin or eosin stains (ie., do I need to fear that there will be sufficient levels of RNAses such that it would be wise to make up my own stain in my lab)?

Thanks for any help,


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