Factor VIII or von Willebrand Factor

<< Previous Message | Next Message >>
From:"Michael J. Lyon, Ph.D." <lyonm@VAX.CS.HSCSYR.EDU>
To:Histonet <HistoNet@Pathology.swmed.edu>
Date:Thu, 08 Apr 1999 15:49:59 -0400
Content-Type:text/plain; charset="iso-8859-1"

Since the original question came on the Histonet I also have a question to
those that have used this antibody.  While my efforts have been successful,
the resulting fluorescence is blotchy within the endothelial cells.  I was
wondering if this is the normal appearance or have I done something wrong.
We would like to use this stain to identify the vascular elements within a
section but also to identify and innervation.  We are using confocal
microscopy so the blotchy staining give discontinuous images.

Any suggestions welcome.


<< Previous Message | Next Message >>