Hi Histonetters,

I'm having trouble getting my Factor VIII (IHC) staining to work for 
me.  I do a straight forward, indirect stain, briefly as follows:

-3% H2O2
-5% normal serum block
-rabbit anti-factor VIII, 60 mins. R.T.
-goat anti-rabbit HRP 60 mins. R.T.
-DAB
-Haematoxylin
(of course wash steps are included in between above)

Is anyone doing anything different that I might try, like ag retreival
perhaps? I'm staining mouse tissues (skin) and they were fixed in 
formalin for 24-48 hours. Any help would be appreciated.
                                                       Cheers! Greg
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851