F4/80 for mouse macrophages.

<< Previous Message | Next Message >>
From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
Date:Tue, 06 Apr 1999 10:20:34 -0600

We do it routinely, but only on frozen sections.  

NBF/paraffin sections generally don't work well with mouse cell surface
markers.  We also grow up our own antibody and stain with the supernate. 
It is available from SeroTec.  

It may work better with Carnoys fixation, instead of NBF.  We avoid 
formalin for all our mouse markers with far better results.  F4/80 is
also known to work with a FORMALIN FREE fixative containing zinc chloride,
zinc acetate, calcium acetate in TRIS buffer then paraffin sectioning.

If you are interested in the frozen section protocol, will be glad to share

Gayle Callis

<< Previous Message | Next Message >>