Cytology preparation

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From:DFlynn9872@aol.com
To:histonet@Pathology.swmed.edu
Reply-To:
Date:Sat, 03 Apr 1999 20:44:18 -0500 (EST)
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Hello Histonetters:

I was curious to find out if other histotechs are also responsible for 
cytology preparation in their laboratories.  And also, if anyone has any 
suggestions on how to do cytoprep on CSF.  I centrifuge the CSF with 
saccomano fluid added for 10 minutes at 3200 rpm (this is the only centrifuge 
I have access to), then cytospin for 3 minutes at 2000 rpm.  The slide is 
then immediately placed in 95% alcohol for fixation until ready to pap stain. 
 The problem is that our pathologists say there are no cells on the stained 
slide.  So I automatically check to see if a cell count has been done, to 
correlate the findings.....of course 90% of them have a ZERO cell count, but 
I am worried about the 10% that shows WBC's and such.

Please help!

Debra Flynn
River Oaks Hospital
Jackson, MS



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