Cutting Tracheas

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From:"Chu, Shirley" <>
Date:Fri, 02 Apr 1999 08:59:05 -0800

I have a colleague that is sectioning OCT embbeded rat tracheas with carotid
arteries attached.  She is having trouble cutting them at around 6-8um in
thickness.  Are there any recommedations or helpful suggestions as to how to
handle sectioning this tissue--temperature, etc?  The tissues were frozen
down in isopentane cooled on liquid nitrogen and she is cutting them on a
Leica cryostat.
Shirley Chu
Valentis Corp
Burlingame CA

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