Cutting Tracheas
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From: | "Chu, Shirley" <schu@megabios.com> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 02 Apr 1999 08:59:05 -0800 |
Content-Type: | text/plain |
I have a colleague that is sectioning OCT embbeded rat tracheas with carotid
arteries attached. She is having trouble cutting them at around 6-8um in
thickness. Are there any recommedations or helpful suggestions as to how to
handle sectioning this tissue--temperature, etc? The tissues were frozen
down in isopentane cooled on liquid nitrogen and she is cutting them on a
Leica cryostat.
Shirley Chu
Valentis Corp
Burlingame CA
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