Re: [Histonet] immuno controls for cell blocks

From:"Richard Cartun"

We use our regular "formalin-fixed, paraffin-embedded" control tissues for all cytology IHC applications (direct smears, Thin-Preps, and cell blocks).


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Vickroy, Jim"  04/21/08 9:27 AM >>>

We are currently using a bank of formalin fixed tissue controls for our immunohistochemical stains.   We have done a short study to show that the reactivity and specificity of our immunostains on the cellblocks, hardening in 95%ETOH and then transferred in formalin, is not different than our regularly formalin fixed tissue.
As I read the new CAP checklist this is what we are required to do.    Now a cytopathologist wants me to maintain a set of controls only for his cellblocks.

How is everybody else handling this situation?

Jim Vickroy
Memorial Medical Center
Springfield, Illinois.

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