Re: [Histonet] Thioflavin S staining

From:Rene J Buesa



If the sections HAVE to be unfixed, you are doing the best you can. IF they can be fixed (NBF for instance) then their integrity will be assured and the staining could be the same.
  Ren J.

"Leskovjan, Andreana"  wrote:
  Hi,



I need to stain 30 um frozen, unfixed mouse brain sections with
Thioflavin S. The sections are mounted onto a very thin plastic film
called Ultralene. I know it's not the best but necessary for our
measurements. I have been using a very simple procedure so far with
minimal chemicals to avoid interference with the measurements: 50%
ethanol for 5 min; 0.0125% Thioflavin S for 3 min; wash in 50% ethanol,
then nanopure water. The staining works however the tissue is almost
destroyed. There are holes, tears, and folds everywhere, which makes
the plaques difficult to see and measure. I've tried many combinations
of the above and this is the best I could get. Does anyone have a
suggestion on how to prevent this and get better looking sections? 



Thanks,

Andreana Leskovjan

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