Re: [Histonet] PT module - antigen retrieval

From:"Thomas Pier"



I've been using it for about a year now.  After the PT Module cools back
to 75 degrees, I remove the racks and rinse the slides with TBS-T that
has been warmed in a 60 degree oven to rinse away any residual paraffin.
 Then, I flood the slides with buffer to keep them from drying out.

Tom

>>> "Taylor, Jean"  05/02/08 2:19 PM >>>
Hi everyone,

I'm testing Lab Vision's PT Module and would like to get feedback from
people currently using this system. I believe it's also the same module
that Dako sells. One concern I have is with the slides drying out before
I have the chance to rinse them. I was always told to never remove
slides from hot buffer solution for this reason. Any other pros or cons
would be helpful.

Thanks!

Jean Taylor, HT(ASCP)QIHC
Meriter Labs
Madison, WI 53715



-----Original Message-----
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[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Friday, May 02, 2008 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 54, Issue 2


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Today's Topics:

   1. Re: p16ink4 (anh2006@med.cornell.edu)
   2. AW: [Histonet] p16ink4 - thanks (Gudrun Lang)
   3. Mouse anti-C5b-9 (Angela Bitting)
   4. Pneumocystis controls (Jennifer Johnson)
   5. Tea Bags (Cindy DuBois)
   6. IF muscle cytoskeletal proteins - interior of myocytes
      doesn't stain (Merced Leiker)
   7. Paraffin types (Joyce Cline)
   8. (no subject) (Lacy, H Marie)
   9. Re: Paraffin types (Gayle Callis)
  10. Re: Paraffin types (Anne van Binsbergen)
  11. Re: Paraffin types (Piero Nelva)
  12. Ventanna Giemsa (Teri.Hallada@midmichigan.org)
  13. RE: Ventanna Giemsa (Mike Pence)
  14. RE: Paraffin types (Weber, Susan (VHACLE))
  15. DRS 60 jackpot! (Tom McNemar)
  16. RE:Pneumocystis controls (Schaundra Walton)
  17. IL-1 IL-6 Tnf alpha help (Orr, Rebecca)
  18. (no subject) (Masterson_John)


----------------------------------------------------------------------

Message: 1
Date: Thu, 1 May 2008 17:25:16 +0000
From: anh2006@med.cornell.edu
Subject: Re: [Histonet] p16ink4
To: "Dana Settembre" ,
	histonet@lists.utsouthwestern.edu
Message-ID:
	<48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe115.bisx.prod.on.blackberry>
	
Content-Type: text/plain

Cell Signaling and Santa Cruz also sells p16 but I have no idea if it
works for IHC.
 
-----Original Message-----
From: Dana Settembre 

Date: Thu, 01 May 2008 12:58:44 
To:Gudrun Lang , histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] p16ink4


Gudrun,
I believe you are correct mtmlab has a patent and is supposed to be 
the only people that sell it.
Cell Marque was given permission to sell what they had by a certain
time, I think.

I too only need the antibody and not the whole kit but they
have you buy their whole kit and it is expensive.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Gudrun Lang  05/01/08 12:48 PM >>>
Hi listmembers,

I am trying to find a vendor for p16ink antibody for cervical cancer
diagnostic. Is it possible, that p16 is only selled by one firm called
mtmlab now?

It looks like they have the patent on the antibody. Or is there any
other
resource?

I need "only" the antibody concentrate and not a whole kit to run it on
the
Ventana benchmark. I found a p16 on the VenAny help is appreciated.

 

Gudrun Lang


_______________________________________________
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Histonet@lists.utsouthwestern.edu
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------------------------------

Message: 2
Date: Thu, 1 May 2008 19:37:38 +0200
From: "Gudrun Lang" 
Subject: AW: [Histonet] p16ink4 - thanks
To: 
Message-ID: <002101c8abb2$0d23d680$eeeea8c0@dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

Thank you all for your answers.

Gudrun Lang
 

-----Urspr√ľngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von
anh2006@med.cornell.edu
Gesendet: Donnerstag, 01. Mai 2008 19:25
An: Dana Settembre; histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] p16ink4

Cell Signaling and Santa Cruz also sells p16 but I have no idea if it
works
for IHC.
 
-----Original Message-----
From: Dana Settembre 

Date: Thu, 01 May 2008 12:58:44 
To:Gudrun Lang , histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] p16ink4


Gudrun,
I believe you are correct mtmlab has a patent and is supposed to be 
the only people that sell it.
Cell Marque was given permission to sell what they had by a certain
time, I think.

I too only need the antibody and not the whole kit but they
have you buy their whole kit and it is expensive.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Gudrun Lang  05/01/08 12:48 PM >>>
Hi listmembers,

I am trying to find a vendor for p16ink antibody for cervical cancer
diagnostic. Is it possible, that p16 is only selled by one firm called
mtmlab now?

It looks like they have the patent on the antibody. Or is there any
other
resource?

I need "only" the antibody concentrate and not a whole kit to run it on
the
Ventana benchmark. I found a p16 on the Ventana website, but cannot
see, if
it is for the same purpose.

Any help is appreciated.

 

Gudrun Lang


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 3
Date: Thu, 01 May 2008 14:09:52 -0400
From: "Angela Bitting" 
Subject: [Histonet] Mouse anti-C5b-9
To: 
Message-ID: <4819CF30.2B7F.00C9.0@geisinger.edu>
Content-Type: text/plain; charset="us-ascii"

Is anyone using this antibody for distinction of lupus vs
dermatomyositis, and would like to share their automated protocol?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!




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N:Bitting;Angela
END:VCARD


------------------------------

Message: 4
Date: Thu, 1 May 2008 14:28:53 -0400
From: Jennifer Johnson 
Content-Type: text/plain; charset="iso-8859-1"


I use Histology Control Systems, Inc for Pneumocystis controls. 
1-800-253-2768 or www.HistologyControlSystems.com
 
Also, thanks to everyone who gave me advice on the Bielschowsky stain. 
One drop of concentrated Hydrochloric acid did NOT yield a positive
result.  You really do need the ONE drop of concentrated Nitric Acid for
the stain to work properly.
 
Jennifer Johnson, HTL (ASCP)
_________________________________________________________________
Make i'm yours.  Create a custom banner to support your cause.
http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN_Make_IM_Yours

------------------------------

Message: 5
Date: Thu, 1 May 2008 11:45:17 -0700
From: "Cindy DuBois" 
Subject: [Histonet] Tea Bags
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<5d9104a30805011145l169a3c8m4c8f539c283ce7b2@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

We bought the tea bags and so far have liked them better than the nylon
bags
for biopsies such as colon, esophagus & gastric.
How are they for ECCs and EMB's?  My concern is the ECC's are usually
very
scant and I am afraid what little there is may be absorbed by the paper
in
the tea bag.  What has been your experience?  In your experience are
they
okay to use for scant specimens?

Cindy


------------------------------

Message: 6
Date: Thu, 01 May 2008 16:55:58 -0400
From: Merced Leiker 
Subject: [Histonet] IF muscle cytoskeletal proteins - interior of
	myocytes doesn't stain
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=us-ascii; format=flowed

Hi,

I'm trying to stain hamster skeletal and cardiac muscle for Troponin  I,

Troponin T, and Myosin Heavy Chain (separately). When I do Z stack
imaging 
at 0.5um steps, only the outermost myofibrils (near the sarcolemma) of
most 
of the myocytes are stained; the interior (particularly of te larger 
myocytes) is unstained, looking like a gaping black hole.

The tissues were snap frozen, cut 8um thick, fixed on the slide in
4%PFA, 
and permeabilized in 1% Triton.  All antibody incubations were done at 
either 1 hr RT or overnight 4oC.  The microscope used was Zeiss
Axioimager 
(epifluorescent).

Can you tell me if this is expected staining for these material and 
conditions, or if something is wrong?

Thank you.

Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725




------------------------------

Message: 7
Date: Thu, 1 May 2008 17:06:57 -0400
From: "Joyce Cline" 
Subject: [Histonet] Paraffin types
To: 
Message-ID: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org>
Content-Type: text/plain;	charset="us-ascii"

What type of paraffin is everyone using? Separate paraffin for
processing and embedding, or the same for processing and embedding?
 
Paraplast, Surgipath, Shandon ????


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------------------------------

Message: 8
Date: Thu, 1 May 2008 17:25:17 -0500
From: "Lacy, H Marie" 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Message-ID: <8520B38B62EC9B43991358FB6732346C408778@MAIL4.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii

Good day everyone,

 

Does anyone have a protocol for the naphthol to use
this stain to identify and count PMNs in sections of guinea pig and
mouse
tissue. I have tried both Sigma kits for this stain (#90 and #91). They
work
well on human tissue but not on guinea pig or mouse (even after varying
the
pH and time).

 

Your help will be greatly appreciated.

 

Marie Lacy

 

 

 


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------------------------------

Message: 9
Date: Thu, 1 May 2008 18:13:23 -0600
From: "Gayle Callis" 
Subject: Re: [Histonet] Paraffin types
To: "Joyce Cline" ,
	
Message-ID: <001201c8abe9$561a8f50$6501a8c0@DHXTS541>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Same paraffin for infiltration and embedding.  Our choice is especially
good 
for bone because it is a harder paraffin and works well for soft tissues

even very thin sections i.g. 1 to 2 um.  Since we do a great deal of
bone 
work, this has been a good choice.    Tissue Prep 2 from ThermoFisher 
Scientific.

We have used the Surgipath combination infiltration media followed by
their 
embedding media with great success.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Joyce Cline" 
To: 
Sent: Thursday, May 01, 2008 3:06 PM
Subject: [Histonet] Paraffin types


> What type of paraffin is everyone using? Separate paraffin for
> processing and embedding, or the same for processing and embedding?
>
> Paraplast, Surgipath, Shandon ????
>
> 




------------------------------

Message: 10
Date: Fri, 2 May 2008 10:59:19 +0400
From: "Anne van Binsbergen" 
Subject: Re: [Histonet] Paraffin types
To: "Gayle Callis" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

one size fits all - tissue-tek works for me - been using it for over 10
years now - in all the labs i have managed
Annie

2008/5/2 Gayle Callis :

> Same paraffin for infiltration and embedding.  Our choice is
especially
> good for bone because it is a harder paraffin and works well for soft
> tissues even very thin sections i.g. 1 to 2 um.  Since we do a great
deal of
> bone work, this has been a good choice.    Tissue Prep 2 from
ThermoFisher
> Scientific.
>
> We have used the Surgipath combination infiltration media followed by
> their embedding media with great success.
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
>
>
> ----- Original Message ----- From: "Joyce Cline" 
> To: 
> Sent: Thursday, May 01, 2008 3:06 PM
> Subject: [Histonet] Paraffin types
>
>
>  What type of paraffin is everyone using? Separate paraffin for
> > processing and embedding, or the same for processing and embedding?
> >
> > Paraplast, Surgipath, Shandon ????
> >
> >
> >
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Anne (van Binsbergen) Hope
Abu Dhabi
UAE


------------------------------

Message: 11
Date: Fri, 2 May 2008 21:12:27 +1000
From: "Piero Nelva" 
Subject: Re: [Histonet] Paraffin types
Cc: 
Message-ID: <004e01c8ac45$67c761e0$5c72be7c@pentium4>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

I've only ever used the samFrom: "Anne van Binsbergen" 
To: "Gayle Callis" 
Cc: 
Sent: Friday, May 02, 2008 4:59 PM
Subject: Re: [Histonet] Paraffin types


> one size fits all - tissue-tek works for me - been using it for over
10
> years now - in all the labs i have managed
> Annie
>
> 2008/5/2 Gayle Callis :
>
>> Same paraffin for infiltration and embedding.  Our choice is
especially
>> good for bone because it is a harder paraffin and works well for soft
>> tissues even very thin sections i.g. 1 to 2 um.  Since we do a great
deal 
>> of
>> bone work, this has been a good choice.    Tissue Prep 2 from 
>> ThermoFisher
>> Scientific.
>>
>> We have used the Surgipath combination infiltration media followed by
>> their embedding media with great success.
>>
>> Gayle M. Callis
>> HTL/HT/MT(ASCP)
>> Bozeman MT 59715
>>
>>
>> ----- Original Message ----- From: "Joyce Cline" 
>> To: 
>> Sent: Thursday, May 01, 2008 3:06 PM
>> Subject: [Histonet] Paraffin types
>>
>>
>>  What type of paraffin is everyone using? Separate paraffin for
>> > processing and embedding, or the same for processing and embedding?
>> >
>> > Paraplast, Surgipath, Shandon ????
>> >
>> >
>> >
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> -- 
> Anne (van Binsbergen) Hope
> Abu Dhabi
> UAE
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> -- 
> No virus found in this incoming message.
> Checked by AVG.
> Version: 7.5.524 / Virus Database: 269.23.7/1410 - Release Date:
5/1/2008 
> 5:30 PM
>
> 




------------------------------

Message: 12
Date: Fri, 2 May 2008 08:14:11 -0400
From: Teri.Hallada@midmichigan.org
Subject: [Histonet] Ventanna Giemsa
To: histonet@pathology.swmed.edu
Message-ID:
	<8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net>
Content-Type: text/plain; charset=us-ascii

We are having trouble with our Ventanna Giemsa stain for bone marrows.
The cells either fall off the slide or explode. Anyone out there willing
to share there procedure or have any suggestions? 

Teresa Hallada BS, MT/CT (ASCP)
Lead Cytotechnologist
MidMichigan Health - Gratiot
teri.hallada@midmichigan.org
989.463.1101 ext 3423

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------------------------------

Message: 13
Date: Fri, 2 May 2008 08:18:42 -0500
From: "Mike Pence" 
Subject: RE: [Histonet] Ventanna Giemsa
To: ,	
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A380D@IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

I could never get this stain to work for us and we went back to manual
staining.
Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Teri.Hallada@midmichigan.org
Sent: Friday, May 02, 2008 7:14 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Ventanna Giemsa


We The cells either fall off the slide or explode. Anyone out there willing
to share there procedure or have any suggestions? 

Teresa Hallada BS, MT/CT (ASCP)
Lead Cytotechnologist
MidMichigan Health - Gratiot
teri.hallada@midmichigan.org
989.463.1101 ext 3423

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privileged and confidential information that is protected against use or
disclosure under federal and state law.  The information is intended
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are hereby notified that you have received this information in error and
that any review, dissemination, distribution, copying or action taken in
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_______________________________________________
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------------------------------

Message: 14
Date: Fri, 2 May 2008 09:56:01 -0400
From: "Weber, Susan (VHACLE)" 
Subject: RE: [Histonet] Paraffin types
To: "Joyce Cline" ,
	
Message-ID:
	<16C83872A53F4346AA9C3A18E3A3AAB903F76D92@VHAV10MSGA1.v10.med.va.gov>
Content-Type: text/plain;	charset="us-ascii"

We are using Surgipath Blue Ribbon Tissue Embedding/Infiltration Medium.
As the name implies, we use only this one paraffin for both.

Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Thursday, May 01, 2008 5:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin types

What type of paraffin is everyone using? Separate paraffin for
processing and embedding, or the same for processing and embedding?
 
Paraplast, Surgipath, Shandon ????


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the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
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------------------------------

Message: 15
Date: Fri, 2 May 2008 11:31:33 -0400
From: "Tom McNemar" 
Subject: [Histonet] DRS 60 jackpot!
To: 
Message-ID:
	<51D5D78FBEDAEA4FBCCD9A9D44211DC528F568@lmhsmail.lmhealth.org>
Content-Type: text/plain;	charset="iso-8859-1"

Rummaging around and low and behold....  I found 10-12 plastic staining
dishes for the Sakura DRS 60!  I know that some of these workhorses are
still around and that you probably can't get dishes for them anymore,
so....

Send me your fedex number and address and I'll ship them out to anyone
who wants them.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org




------------------------------

Message: 16
Date: Fri, 2 May 2008 08:48:53 -0700 (PDT)
From: Schaundra Walton 
Subject: [Histonet] RE:Pneumocystis controls
To: Histonet 
Message-ID: <661422.27682.qm@web58909.mail.re1.yahoo.com>
Content-Type: text/plain; charset="us-ascii"


   We just recently ordered these controls slides from Mercedes Medical.
   [1]www.mercedesmedical   and Mercedes' prices were pretty comparable.


   Hope this helps!


   Schaundra Walton BS, HTL(ASCP)

   Histology Supervisor

   Swedish American Hospital

   Rockford, IL


   -----Original Message-----


   Hoping someone knows of a supplier of pneumocystis TISSUE control
   slides?
   Thanks
   Nancy Rutledge
     _________________________________________________________________

   Be  a  better  friend,  newshound, and know-it-all with Yahoo!
Mobile.
   [2]Try it now.

References

   1. http://www.mercedesmedical.com/
   2.
http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ


------------------------------

Message: 17
Date: Fri, 2 May 2008 11:01:28 -0500
From: "Orr, Rebecca" 
Subject: [Histonet] IL-1 IL-6 Tnf alpha help
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I everyone,

I have a researcher who is looking for a lab that will run these
antibodies on 24hr Urines.

I'm not sure how many samples he has, but if anyone has a reference lab
they could recommend I'd be obliged.

Mayo does some of these but only on blood/plasma.

I'm thinking the specimen type will be the issue.

 

Thanks and let's all have a great weekend.

 

 

Becky Orr CLA,HT(ASCP)QIHC

Anatomic Pathology

Evanston Northwestern Healthcare

847-570-2771

 



------------------------------

Message: 18
Date: Fri, 2 May 2008 09:30:57 -0700
From: "Masterson_John" 
Subject: [Histonet] (no subject)
To: "Orr, Rebecca" , 
Message-ID:
	<0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com>
	
Content-Type: text/plain;	charset="us-ascii"



-----Original Message-----
Hi Histonetter's,

Thanks to all who replied to my last question regarding M.O.M. IHC kits.
Does anyone have any wisdom regarding the Bodian stain.  I've purchased
a kit from a well known vendor but after 4 or 5 attempts I still can't
get the section to turn dark tan to brown as described for the protargol
step. I've ordered a bottle of pre mixed 1% protargol from another
vendor and it doesn't work any better.  Any advice?

Thanks,

John





------------------------------

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End of Histonet Digest, Vol 54, Issue 2
***************************************

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