From: | "Joanne Mauger" |
Nancy, Weuse HIER with citrate buffer, pH 6.0- should translate to the ER1 buffer on the Bond. Dilution we use with Envision+ is 1:100. I have diluted most antibodies out further with the Bond detection-try 1:200. Jo Mauger Children's Hospital of Philadelphia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet |