I have been using the same sequence of reagents for several years with
great success. We routinely fix bone marrow cores for 3 hours in B-plus,
rinse in water, decalcify, rinse again, and put the cassette in with all
the other tissues for processing. B-plus contains formaldehyde anyway,
so your are not introducing a different reagent when you transfer them
to formalin during processing.
B-plus gives much better cytological detail than formalin. Nuclear
detail is crisper, granules are better preserved, hemosiderin is not
effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after
B-plus. It also has the advantage of not producing an fixation artefact
pigment like B-5 does. I think you will be happy with your decision to
karen adams wrote:
> Hello all....we are changing bone marrow fixative from formalin to B-plus.
> If we fix for the required time in B-plu and then process on the VIP w/ the
> other specimens using formalin are there any effects on the tissue going
> from B-plus to formalin??
> Thank you in advance
Histonet mailing list