RE: [Histonet] validation of lot #'s for IHC & Specials

From:"Sebree Linda A."


We pretty much do something similar as you for IHC control lot
verification.  If we've exhausted a block currently in use as a control,
we'll select a new tissue and stain it with the old lot and new lot.
Obviously this takes some planning ahead but you'd never want to run out
of your old lot of antibody before you have some new on hand.  We've
just moved to a new system whereas we maintain 1 soft tissue control and
1 bm bx control (if applicable) for routine use.  However we have on
hand  several control tissues to check out new lots of antibody.  We
feel this is a "safer" method of lot verification using controls of
varying degrees of immunoreactivity.  I hope this isn't too confusing.
If you have further questions, feel free to contact us.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
[] On Behalf Of Joyce
Sent: Tuesday, April 29, 2008 11:00 AM
Subject: [Histonet] validation of lot #'s for IHC & Specials

We currently pull a control already stained with the old lot # and stain
a control with the new lot # and compare staining. Our paths are saying
you should use the same control block for both the old and new lot #'s
but we don't always have the same block (might have been exhausted)
Does anyone keep a control block without exhausting it, for lot
Does anyone check lot #'s for their special stains. We have a Ventana
NexES Sp. Stainer and we order pre-made kits for our hand staining. Our
paths want us to validate the lot #'s for our specials also.
Thanks..Joyce :-)

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