RE: [Histonet] immuno controls for cell blocks

From:Rene J Buesa



Jim:
  Your validation is adequate and you don't need to prepare a new set of (+) cell blocks. I think this CT will be smart enough to understand what a validation process is all about and that CAP is OK with that. It is always easy to ask, when you don't know what the answer involves!
  René J.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Monday, April 21, 2008 8:27 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] immuno controls for cell blocks



We are currently using a bank of formalin fixed tissue controls for our
immunohistochemical stains. We have done a short study to show that
the reactivity and specificity of our immunostains on the cellblocks,
hardening in 95%ETOH and then transferred in formalin, is not different
than our regularly formalin fixed tissue.
As I read the new CAP checklist this is what we are required to do.
Now a cytopathologist wants me to maintain a set of controls only for
his cellblocks.

How is everybody else handling this situation?

Jim Vickroy
Memorial Medical Center
Springfield, Illinois.



       
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