This has always been confusing, but, if I understand correctly, the
negative control is the same patient tissue without primary antibody.
Use a negative for each detection system used if you have multiple
antibodies on the same block.
Joyce
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, April 30, 2008 1:05 PM
To: Sarah Kolekamp; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Control Tissue
I think you would have to use the same tissue you use for your + control
to show that it "is working/staining" and "is not working/staining"
At least that is how we do it.
Mike
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah
Kolekamp
Sent: Wednesday, April 30, 2008 11:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Control Tissue
I have a questions about negative control tissue, does anyone know what
the most universal tissue would be that we could use to act as our
negative control tissue. We are looking for the tissue that could be
used with the widest array of antibodies.
Thanks!
Sarah Kolekamp HTL (ASCP)
Lead Histotechnologist
Saint Mary's Health Care
Phone Number (616) 752-6155
Fax Number (616) 774-0628
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