We have this happen when the chuck is the temperature of the cryostat before we put the OCT on it. The OCT freezes before it has a chance to seep into the grooves. Try putting the OCT on the warm chuck just after you put the chuck on the bar in the cryostat.
From: firstname.lastname@example.org on behalf of Webb, Dorothy L
Sent: Wed 5/7/2008 1:56 PM
Subject: [Histonet] Lot numbers
Does everyone keep track of each reagent lot number, including, water,
formalin, alcohols, etc. as it is opened and used? What about recycled
products? I am trying to come up with a logsheet and would appreciate
any help in this area!
Also, we have recently run into problems with our freezing compound
coming off of the chucks during frozen sectioning. Any suggestions
and/or what is the method of freezing tissue that everyone uses for
their cryostat sections? Thanks fellow histotechs for helping me with
these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!!
Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Regions Hospital is part of the HealthPartners family of care
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.
Histonet mailing list
The information contained in this message may be privileged and/or confidential
and protected from disclosure. If the reader of this message is not the intended
recipient or an employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any dissemination, distribution
or copying of this communication is strictly prohibited. If you have received this
communication in error, please notify the sender immediately by replying to this
message and deleting the material from any computer.
Histonet mailing list