We have this happen when the chuck is the temperature of the cryostat before we put the OCT on it. The OCT freezes before it has a chance to seep into the grooves. Try putting the OCT on the warm chuck just after you put the chuck on the bar in the cryostat.
From: email@example.com on behalf of Webb, Dorothy L
Sent: Wed 5/7/2008 1:56 PM
Subject: [Histonet] Lot numbers
Does everyone keep track of each reagent lot number, including, water,
formalin, alcohols, etc. as it is opened and used? What about recycled
products? I am trying to come up with a logsheet and would appreciate
any help in this area!
Also, we have recently run into problems with our freezing compound
coming off of the chucks during frozen sectioning. Any suggestions
and/or what is the method of freezing tissue that everyone uses for
their cryostat sections? Thanks fellow histotechs for helping me with
these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!!
Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Regions Hospital is part of the HealthPartners family of care
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