RE: [Histonet] Hepatocyte Specific Antigen

From:"Sebree Linda A."


We're using Biocare's HSA (CM 166), 1:50, on our Ventana instruments but
do use HIER.  Off-line we use a high pH Tris buffer for 2" in a lab
pressure cooker and on-line we use Ventana's CC1 which is also a Tris
buffer.  We achieve beautiful, clean staining using these protocols.
Maybe you can adapt this to your Bond stainer.

Good luck,

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
[] On Behalf Of Nancy
Sent: Monday, April 21, 2008 11:00 AM
To: ''
Subject: [Histonet] Hepatocyte Specific Antigen

Hello histonetters - 

Working up Hepatocyte marker on BOND stainer.  Not really getting the
results we wanted.  Calls for no pretreatment - is anybody doing

Thanks for your help

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
Dubuque, IA

NOTICE: This email may contain legally privileged information. The
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the
that you have received it in error and then delete it along with any
attachments. Thank you.

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>