We tried the new Surgipath staining system, and while I was very
happy with the stains, I found the Define to be ineffective at
removing all of the background staining. So, I kept the Hematoxylin
and Eosin/Phloxine and continued to use old-fashioned 1%HCl in 70%
ethanol to clarify and Scott's tap water substitute to blue.
Staining looks great, no need to filter Hematoxylin, and the stains
are still working when I discard after ~2000 slides.
Facility Manager Histology
David H. Koch Institute for Integrative Cancer Research
Massachusetts Institute of Technology
40 Ames St. E17-427
Cambridge MA 02139
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