Good day everyone,

 

Does anyone have a protocol for the naphthol AS-D chloroacetate esterase
stain for formalin fixed, paraffin-embedded animal tissue? I am trying to use
this stain to identify and count PMNs in sections of guinea pig and mouse
tissue. I have tried both Sigma kits for this stain (#90 and #91). They work
well on human tissue but not on guinea pig or mouse (even after varying the
pH and time).

 

Your help will be greatly appreciated.

 

Marie Lacy

 

 

 


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