Msg 20: H&E question (crochieresteve@aol.com)
Did you try a 2 minute soak of slides in 250mL 1xPBS + 1mL 100% Conc.
HCl after your Hematoxylin stain? Then run the slides gently under
slowly running H20 (can be dDH20 or tap) for one minute and continue on
with dehydrating and staining.
>>> 04/24/08 1:01 PM >>>
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
1. Automated Special Stainers (Victor Tobias)
2. Cornea nerve fibers (Paula Pierce)
3. RE: Automated Special Stainers (Houston, Ronald)
4. Free workshop (Shirley Powell)
5. IGF-IR antibody (Martha Ward) (Jean-Martin Lapointe)
6. RE: Automated Special Stainers
(Bartlett, Jeanine (CDC/CCID/NCZVED))
7. Paraformaldehyde use question for geniuses (Jackie M O'Connor)
8. Re: Paraformaldehyde use question for geniuses (Geoff McAuliffe)
9. Grossing Qualifications (thisisann@aol.com)
10. IHC Staining Variations (Margaryan, Naira)
11. Re: Automated Special Stainers (Victor Tobias)
12. opportunity in Honduras (suboro)
13. unsubsribe (Kathy Gorham)
14. VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues
(Cathy Malcontenti-Wilson)
15. Re: Cornea nerve fibers (John Kiernan)
16. RECA-1 (Helen Ilsley)
17. Exciting management opportunity (Pam Barker)
18. HT qualifications (Cheri Miller)
19. pH probes and dyes-compatible? (Greg Dobbin)
20. H&E question (crochieresteve@aol.com)
----------------------------------------------------------------------
Message: 1
Date: Wed, 23 Apr 2008 10:19:54 -0700
From: Victor Tobias
Subject: [Histonet] Automated Special Stainers
To: Histonet
Message-ID: <480F6FBA.1000303@pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Our PA who oversees Grossing and Histology asked me to post a question
to the group. We currently have the Artisan (about 5 years d)
and would like to know if there is something better for quality and
output.
Thanks
Victor
--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-598-2792
206-598-7659 Fax
==========================3D=======================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.
------------------------------
Message: 2
Date: Wed, 23 Apr 2008 10:21:41 -0700 (PDT)
From: Paula Pierce
Subject: [Histonet] Cornea nerve fibers
To: Histonet
Message-ID: <996977.16027.qm@web50104.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
áThe corneaáonly has unmyelinatedánerve fibers, so a luxol fast blue
will not work. Try Rene's suggestion.
áPaula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway Ave.
Moore, OK 73160
405-759-3953 lab
contact@excaliburpathology.com
www.excaliburpathology.com
------------------------------
Message: 3
Date: Wed, 23 Apr 2008 13:24:29 -0400
From: "Houston, RonSubject: RE: [Histonet] Automated Special Stainers
To: "Victor Tobias" , "Histonet"
Message-ID:
<979FF5962E234F45B06CF0DB7C1AABB20FEC1F46@chi2k3ms01.columbuschildrens.net>
Content-Type: text/plain; charset="us-ascii"
Nothing comes close as far as quality is concerned
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5450
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor
Tobias
Sent: Wednesday, April 23, 2008 1:20 PM
To: Histonet
Subject: [Histonet] Automated Special Stainers
Our PA who oversees Grossing and Histology asked me to post a question
to the group. We currently have the Artisan (about 5 years d)
and would like to know if there is something better for quality and
output.
Thanks
Victor
--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-598-2792
206-598-7659 Fax
==========================3D=======================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
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if the message has been addressed to you in error, do not read,
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------------------------------
Message: 4
Date: Wed, 23 Apr 2008 13:21:43 -0400
From: Shirley Powell
Subject: [Histonet] Free workshop
To: histonet@lists.utsouthwestern.edu
Message-ID: <01MTYRHM02P2002IFU@Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii
Hi Guys,
Here is a free workshop with free CEUs for those in the Southeast who
can
get to Atlanta on May 21st.
For more information go to our web site, GSH at
www.hiistosearch.com/gsh,
click on the education page
or click on this link.
http://www.histosearch.com/gsh/LeicaSymposium.pdf
Shirley Powell
GSH Secretary
------------------------------
Message: 5
Date: Wed, 23 Apr 2008 14:19:39 -0400
From: "Jean-Martin Lapointe"
Subject: [Histonet] IGF-IR antibody (Martha Ward)
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
I have no recent experience with IGF-1R IHC, but for such markers of
cell function, in the past Cell Signaling has always been my first
cho
__________________________________
Jean-Martin Lapointe
------------------------------
Message: 6
Date: Wed, 23 Apr 2008 14:16:18 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)"
Subject: RE: [Histonet] Automated Special Stainers
To: "Houston, Ronald" ,
"Victor Tobias" , Histonet
Message-ID:
<1CE1847DFEA0A647B1CCDE4108EA60A747A23E@LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
We really like our Artisan.....
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston,
Ronald
Sent: Wednesday, April 23, 2008 1:24 PM
To: Victor Tobias; Histonet
Subject: RE: [Histonet] Automated Special Stainers
Nothing comes close as far as quality is concerned
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5450
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor
Tobias
Sent: Wednesday, April 23, 2008 1:20 PM
To: Histonet
Subject: [Histonet] Automated Special Stainers
Our PA who oversees Grossing and Histology asked me to post a question
to the group. We currently have the Artisan (about 5 years d)
and would like to know if there is something better for quality and
output.
Thanks
Victor
--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-598-2792
206-598-7659 Fax
==========================3D=======================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.
_______________________________________________
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----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the sole
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privileged information. The recipient is responsible to maintain the
confidentiality of this information and to use the information only for
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hereby notified that any review, use, disclosure, distribution, copying,
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 23 Apr 2008 14:27:37 -0500
From: Jackie M O'Connor
Subject: [Histonet] Paraformaldehyde use question for geniuses
To: Histonet ,
histonet-bounces@lists.uContent-Type: text/plain; charset="US-ASCII"
Gotcha. How long can tissues fixed in 4% PFA stay in PFA? If I
can't
process them for awhile, what should they be transferred to?
Anyone know if 4%PFA is commercially available in vast quantities?
Jackie O'
------------------------------
Message: 8
Date: Wed, 23 Apr 2008 15:42:01 -0400
From: Geoff McAuliffe
Subject: Re: [Histonet] Paraformaldehyde use question for geniuses
To: Jackie M O'Connor
Cc: Histonet ,
histonet-bounces@lists.utsouthwestern.edu
Message-ID: <480F9109.3060906@umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi Jackie:
A few weeks will be OK. I would leave them in fix rather than 70%
ethanol. I'm sure someone will sell you vast quantities of 4% PFA if you
ask and have $$.
Geoff
Jackie M O'Connor wrote:
> Gotcha. How long can tissues fixed in 4% PFA stay in PFA? If I
can't
> process them for awhile, what should they be transferred to?
> Anyone know if 4%PFA is commercially available in vast quantities?
>
> Jackie O'
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************
------------------------------
Message: 9
Date: Wed, 23 Apr 2008 16:15:43 -0400
From: thisisann@aol.com
Subject: [Histonet] Grossing Qualifications
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CA7395F1657E24-1720-2DF7@WEBMAIL-MC20.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Can someone tell me where I can find CAP's grossing qualifications.? I
need to know what degree and/or specific college courses need to be met
in order to be qualified to gross (grandfather clause as well).
Thank You,
Ann Angelo
AAngelo@QDxPath.com
------------------------------
Message: 10
Date: Wed, 23 Apr 2008 16:53:35 -0500
From: "Margaryan, Naira"
Subject: [Histonet] IHC Staining Variations
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Trinity,
Are you using a Lab Vision stainer? If so I am always having this
problem, even I programmed 3-drop zones with 200ul of reagent dropping
in each zone, for a total of 600ul per slide just to be sure that slide
fully covered. We invited their tech several times. He checked run,
clean and still sometimes I still have this kind of Staining Variations.
I never had this problem before when used DAKO stainer.
Please, let me know when and how did you fixed your problems.
Good luck,
Naira
Naira V. Margaryan, D.V.M., Ph.D.
Research Scientist
Children's Memorial Research Center
2300 Children's Plaza, Box 222
Chicago, IL 60614-3363
Tel: 773-755-6340
Fax: 773-755-6594
nmargaryan@childrensmemorial.org
For Express Mail:
CMRC, Room C.473
2430 N. Halsted Street
Chicago, IL 60614-4314
------------------------------
Message: 11
Date: Wed, 23 Apr 2008 15:22:12 -0700
From: Victor Tobias
Subject: Re: [Histonet] Automated Special Stainers
To: "Bartlett, Jeanine (CDC/CCID/NCZVED)"
Cc: Histonet
Message-ID: <480FB694.1090506@pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Thank you all for the quick responses, looks like we won't be switching
any time soon.
Victor
Victor Tobias
Clinical Applications Analyst
University206-598-2792
206-598-7659 Fax
==========================3D=======================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.
Bartlett, Jeanine (CDC/CCID/NCZVED) wrote:
> We really like our Artisan.....
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett@cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Houston,
> Ronald
> Sent: Wednesday, April 23, 2008 1:24 PM
> To: Victor Tobias; Histonet
> Subject: RE: [Histonet] Automated Special Stainers
>
> Nothing comes close as far as quality is concerned
>
>
>
> Ronnie Houston, MS HT(ASCP)QIHC
>
> Anatomic Pathology Manager
>
> Nationwide Children's Hospital
>
> 700 Children's Drive
>
> Columbus, OH 43205
>
> (614) 722 5450
>
> ronald.houston@nationwidechildrens.org
>
> www.NationwideChildrens.org
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor
> Tobias
> Sent: Wednesday, April 23, 2008 1:20 PM
> To: Histonet
> Subject: [Histonet] Automated Special Stainers
>
>
>
> Our PA who oversees Grossing and Histology asked me to post a question
>
> to the group. We currently have the Artisan (about 5 years d)
>
> and would like to know if there is something better for quality and
> output.
>
>
>
> Thanks
>
> Victor
>
>
>
>
------------------------------
Message: 12
Date: Wed, 23 Apr 2008 15:26:20 -0700 (PDT)
From: suboro
Subject: [Histonet] opportunity in Honduras
To: histonet@lists.utsouthwestern.edu
Message-ID: <738448.37350.qm@web34702.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello, 'Netters!
A medical outreach group that is working in Honduras has an temporary
(two month) position for a histo tech in beautiful Santa Rosa. This is a
great opportunity for someone retired, between jobs, or with a ton of
vacation time, to do some good in the world, have a great vacation, and
earn some lempiras all at the same time.
Please contact me for more information
Susan Borocz HT(ASCP)
suboro@yahoo.com
---------------------------------
Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try
it now.
------------------------------
Message: 13
Date: Wed, 23 Apr 2008 16:15:59 -0700
From: "Kathy Gorham"
Subject: [Histonet] unsubsribe
To:
Message-ID: <000601c8a597$fe83bbb0$2f01a8c0@kathy83b707eca>
Content-Type: text/plain; charset="iso-8859-1"
------------------------------
Message: 14
Date: Thu, 24 Apr 2008 11:05:19 +1000
From: Cathy Malcontenti-Wilson
Subject: [Histonet] VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues
To: Histonet@lists.utsouthwestern.edu
Message-ID:
<452655D3CC46464485FE5107C8723F2A020487CD@IS-EX-BEV1.unimelb.edu.au>
Content-Type: text/plain; charset="us-ascii"
Dear All,
I would very much appreciate some help from someone who is doing/has
done some immunostaining of FFPE mouse tissues with an antibody against
VEGF Receptor 1/Flt-1. We are currently having difficulties believing
the staining we are getting with the protocols we have tried.
The antibody we have is Santa Cruz Flt-1 c-17 (sc-316).
I would be grateful for any help.
Thanks
Cathy
------------------------------
Message: 15
Date: Thu, 24 Apr 2008 0To: "Yaskovich, Ruth A (NIH/NIDCR) [E]"
Cc: Histonet
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Dear "Yaskovich, Ruth A (NIH/NIDCR) [E]"
The technical issu preparation: sections or
whole-mounts. A cornea curved in 3D and it resists pressing to
make a flat spe
John Kiernan
Anatomy, UWO
Londo
Canada
----- Original Message -----
From: "Yaskov ich, Ruth A
(NIH/NIDCR) [E]"
Subject
nerve fibers
To: Histonet
> Hi all,
doe cornea?
>
> Ruth Yaskovich
National
Institutes of Health
>
>
> _______
_______________________ 5F Histone
Histonet@lists.utsouthwestern.edu<
http://lists.utsouthwestern.edu/mailman/listinfo/his tonet
------------------------------
Message: 16
Date: Thu, 24 Apr 2008 07:26:02 +0200
From: Helen Ilsley
Subject: [Histonet] RECA-1
To: histonet@lists.utsouthwestern.edu
Message-ID: <9E250B99-D222-455D-9B63-75CF16536297@uct.ac.za>
Content-Type:
text/plain; charset=US-ASCII; delsp=yes; format=flowed
Hi
I wonder if anyone out there is using RECA-1 on FFPE tissue using a
fluorescent method.
I am using it on rat heart. I can get it working on light but am
battling to get a signal on IF.
I would really appreciate it if anyone out there has managed to get
this working for IF and could email me their protocol.
Many thanks
Helen
Helen Ilsley
Helen.Ilsley@uct.ac.za
Cardiovascular Research Unit
Cape Heart Centre
Anzio Road, Observatory, UCT
021-406 6398/6590
021-448 5935 (fax)
------------------------------
Message: 17
Date: Thu, 24 Apr 2008 09:30:01 -0400
From: "Pam Barker"
Subject: [Histonet] Exciting management opportunity
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters!
I have a management position that I want to tell you about. This is a
permanent full time Histology Supervisor position with a prestigious
facility in the San Francisco Bay Area. This position is in a clinical
environment. My client is looking for someone with a minimum of 5 years
of
experience in immunohistochemistry and histology supervision along with
ASCP HT/HTL and the QIHC certification is preferred. My client offers
excellent compensation and benefits. If you or someone you know might
be
interested in hearing more about this position please contact me.
Incidentally I also have exciting management opportunities in Portland,
OR,
Los Angeles, CA and Dallas, TX and bench positions in CA, FL, TX, PA,
VA, MD
and WA.
Have a great day!!
Thank You!
Pam M. Barker
President
Relia
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
Toll Free: (866)607-3542
e-mail: relia1@earthlink.net
http://home.earthlink.net/~relia1
www.myspace.com/pamatrelia
------------------------------
Message: 18
Date: Thu, 24 Apr 2008 10:23:06 -0500
From: "Cheri Miller"
Subject: [Histonet] HT qualifications
To:
Message-ID: <000001c8a61f$1900d0e0$3d02a8c0@plab.local>
Content-Type: text/plain; charset="us-ascii"
I want to just get verification from the experts. I have an employee in
Cyto
-prep that wants to get into histology. She went to the local collage
got
information on obtaining an Associate in Science Degree. With that and 1
year of practical (OJT) in a histology laexam, correct? I currently have 2 OJT techs I am training but they have
a
BA in the sciences already. I just wanted your opinions before I tell
her to
go ahead and enroll.
Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.
If you are not the addressee intended / indicated or agent responsible
for delivering it to the addressee, you are hereby notified that you are
in possession of confidential and privileged information. Any
dissemination, distribution, or copying of this e-mail is strictly
prohibited. If you have received this message in error, please notify
the sender immediately and delete this email from your system.
------------------------------
Message: 19
Date: Thu, 24 Apr 2008 12:26:17 -0300
From: "Greg Dobbin"
Subject: [Histonet] pH probes and dyes-compatible?
To:
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hi Folks,
I will need to use a pH meter in our Chemistry Lab to pH alizarin red.
I would hate to ruin someone else's pH meter! Can anyone tell me if the
repeated use of a pH probe for pH'ing dyes will affect the porosity of
the glass probe or have any other detrimental affects for that matter?
(I would use a pH meter far too infrequently to warrant maintaining one
in my own lab.)
Thanks.
Greg
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
-------------------------
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-------------------------
------------------------------
Message: 20
Date: Thu, 24 Apr 2008 11:43:23 -0400
From: crochieresteve@aol.com
Subject: [Histonet] H&E question
To: histonet@pathology.swmed.edu
Message-ID: <8CA743910799B39-125C-400@webmail-da20.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
I am having issues with the surgipath 560mx hematoxylin. Goblet cells
and mucin are staining blue. I have lowered the time to 1 min in
Hematoxylin followed by 1 min in define and 1 min in bluing with wash
steps in between, but the sections are still giving an over stained
appearance. Would adding acetic acid to lower the pH help with this or
should I try something different?
steve
------------------------------
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End of Histonet Digest, Vol 53, Issue 34
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