I have some questions with the usage of positive RNA probe in the
usage of EBV detection. I was told to do positive RNA probe together
with EBER, kappa and lambda ISH on the test tissue in addition to
positive controls to make sure that the RNA is properly preserve on
the fixation provcess.
Here the problems is do they all need to be the same protocol
for every probes as my kappa, lambda and EBER are using different
hybridization temperature and time?
Please kindly advice.
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