Are you using a Lab Vision stainer? If so I am always having this
problem, even I programmed 3-drop zones with 200ul of reagent dropping
in each zone, for a total of 600ul per slide just to be sure that slide
fully covered. We invited their tech several times. He checked run,
clean and still sometimes I still have this kind of Staining Variations.
I never had this problem before when used DAKO stainer.
Please, let me know when and how did you fixed your problems.
Naira V. Margaryan, D.V.M., Ph.D.
Children's Memorial Research Center
2300 Children's Plaza, Box 222
Chicago, IL 60614-3363
For Express Mail:
CMRC, Room C.473
2430 N. Halsted Street
Chicago, IL 60614-4314
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