Trinity,

 

Are you using a Lab Vision stainer? If so I am always having this
problem, even I programmed 3-drop zones with 200ul of reagent dropping
in each zone, for a total of 600ul per slide just to be sure that slide
fully covered. We invited their tech several times. He checked run,
clean and still sometimes I still have this kind of Staining Variations.
I never had this problem before when used DAKO stainer. 

 

Please, let me know when and how did you fixed your problems.

 

Good luck,

Naira

 

Naira V. Margaryan, D.V.M., Ph.D.

Research Scientist

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3363

Tel: 773-755-6340

Fax: 773-755-6594

nmargaryan@childrensmemorial.org
 

 

For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314

 

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