I am having issues with the surgipath 560mx hematoxylin. Goblet cells and mucin are staining blue. I have lowered the time to 1 min in Hematoxylin followed by 1 min in define and 1 min in bluing with wash steps in between, but the sections are still giving an over stained appearance. Would adding acetic acid to lower the pH help with this or should I try something different?
steve
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