[Histonet] Fluorescein labeling protocol for sheep

From:"Fredericks, Douglas"

I would like to know it any of you have fluorescein labeled sheep and
what protocol is good to follow?  I currently use this one, but I do not
know if it is the best.


1.      Oxytetracycline (30mg/kg IV) at xx weeks

2.      Alizarin Red S (30mg/kg IV) at xx weeks

3.      Fluorescein DCAF (20mg/kg IV) at xx weeks 

4.      Xylenol orange (90mg/kg IV) at xx weeks 


I also would like to know the proper protocol to make these labels.


Suggestions please...







Douglas C. Fredericks


Bone Healing Research Lab

Iowa Spine Research Lab

Department of Orthopaedic Surgery and Rehabilitation

University of Iowa Carver College of Medicine

100 Oakdale Campus #259 ORB

Iowa City, IA 52242

(319) 335-4377

(319) 335-4378  Fax



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