Re: [Histonet] tissue processing

From:Rene J Buesa

If you are using a tissue processor (TP) you do not have to worry about this problem but since you asked it seems that you are going to process manually.
  If that is the case you should maintain the same volume ratio of 20:1 as when fixing.
  The other thing (if processing tissue manually) is that you will not have agitation, pressure, vacuum or controlled temperature, so time has to be increased (as compared with an automatic TP) for each step.
  Volume alone will not determine proper processing without the other advantages of a TP and these disadvantages have to be compensated with longer steps.
  René J. wrote:

Is there a standard tissue volume to liquid volume ratio that you use when 
dehydrating samples with ethanol?
Thank you in advance.

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