Re: [Histonet] alcian blue_ precipitation

From:Gayle Callis



Our pH 2.5 alcian blue was dissolved in 100 ml of 3% glacial acetic acid, 
and pH 1 alcian blue was dissolved in 0.1N HCL.

Your alcian blue may be old?  Or not biological stain commission 
certified.  We also store our alcian blue in the refrigerator, per 
recommendation of R.W. Mowry of McManus and Mowry fame, his lecture at NSH 
some years ago.

At 07:22 AM 4/17/2007, you wrote:
>dear histonetters
>
>   When i try to dissolve the alcian blue (sigma) powder in acidified 
> (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 
> alchol:distilled water adjusted to required pH its dissolving but isnt 
> staining any of mucin cells in positive control. please help
>
>   raghul
>   Medbiotech res. limited
>   chennai, India
>
>
>---------------------------------
>  Check out what you're missing if you're not on Yahoo! Messenger
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>