Re: [Histonet] alcian blue_ precipitation

From:Gayle Callis

Our pH 2.5 alcian blue was dissolved in 100 ml of 3% glacial acetic acid, 
and pH 1 alcian blue was dissolved in 0.1N HCL.

Your alcian blue may be old?  Or not biological stain commission 
certified.  We also store our alcian blue in the refrigerator, per 
recommendation of R.W. Mowry of McManus and Mowry fame, his lecture at NSH 
some years ago.

At 07:22 AM 4/17/2007, you wrote:
>dear histonetters
>   When i try to dissolve the alcian blue (sigma) powder in acidified 
> (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 
> alchol:distilled water adjusted to required pH its dissolving but isnt 
> staining any of mucin cells in positive control. please help
>   raghul
>   Medbiotech res. limited
>   chennai, India
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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