I've just covered a similar issue, but I was given the acronym ATPEs to
search for.... As far as I am now aware, both ATPEs and TESPA refer to
Silane, officially known as 3-aminopropyl-triethoxysilane. You can get
it from Sigma (Cat Ref: A-3648), however, be aware that you can also buy
pre-coated slides - Superfrost Plus - that are already coated with
silane.... If you do some searching of the Histonet archives, you will
turn up many posts debating the pros and cons of Superfrost Plus versus
self-coated silane slides! I'll leave it to you to work out which
option everyone considers the better slide (I've decided to use the
Superfrost Plus for my ISH to begin with and see how good they are)!
I hope that helps.
On 20/04/2007 6:59 PM, Lewis, Sarah wrote:
> I have a doc that is looking for a product called TESPA? Evidently it is
> used to coat slides. The only reference I can find on it is in the
> Histonet archives from 1999. Is anyone familiar with this product? If
> so do you know where I can order it?
> Thanks so much,
> Sarah E. Lewis
> Childrens Research
> Center for Gene Therapy
> 700 Childrens Dr Rm WA3112
> Columbus OH 43205
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