Re: [Histonet] IHC & Special Stains combined

From:Rene J Buesa

  It will have to depend on the special stain (HC) you are going to do. If the "target tissue" for both HC and IHC is the same, they will interfere. If you just want it as a background color there will be no problem.
  The thing is that I do not see the usefulness of that combination.
  Usually IHC targets cells or parts of cells in a very specific way, and HC is used more to stain microorganisms in tissues, or cells in their general tisular arrangement.
  It could be done if the interference is minimal, but I don't see the advantage of submitting a section to the mechanical "stress" of doing HC and IHC on it.
  Just a thought though!
René J.
Sarah Jones  wrote:
  Does anyone out there is Histonetland have any experience with combining
IHC and Special Stains? Any thoughts on this?


Sarah A. Jones, HTL (ASCP) CM

Research Associate, R&D

Artisan Special Stains

Dako North America, Inc.

6392 Via Real

Carpinteria, CA 93013

Tel (800) 235-5743 Ext 5681 

Fax (805) 684-1372

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