As far as I know Herceptin (trastuzumab) is a HUMANIZED monoclonal antibody and not a mouse or rabbit monoclonal. While certainly many things are possible, I'm skeptical of rabbit monoclonals, no matter how great they are for IHC, going into human therapeutics in light of the norm which is to humanize these reagents. Unless you were to "humanize" the rabbit monoclonal itself similar to humanizing mouse monoclonals.
-------------- Original message ----------------------
> I agree that the epitomics website is great for learning about this technology
> and I further agree it could be of help as has been mentioned before. I am a
> bit skeptical of claims of a 10-fold better affinity than mouse monoclonals as a
> blanket statement.
> At a previous meeting a while back we asked about claims of higher affinity and
> couldn't get a sufficient (to our mind) response. To me that response would be
> in the form of a side to side comparison, using the same immunogen, and with a
> host mouse and rabbit side by side each producing monoclonals to same target to
> see which might be better. I believe the concept of a superior rabbit
> monoclonal technology in terms of being able to break immunological tolerance in
> the immunized systems, especially for some difficult targets, is probably right
> But simply breaking tolerance is not the same as producing higher affinity
> For the blanket statement that rabbit monoclonals have higher affinity, I'd like
> to see data comparing them to their (identical) target in a mouse host and see
> data such as from Biacore, x-ray crystallography and that differences in somatic
> hypermutation are indeed making these higher affinity.
> That being said, I agree that the rabbit monoclonals have great use and even
> more potential. I've used several (many) that are far superior to their
> counter-part mouse monoclonals. However, I've used (and heard of) a few that
> weren't as good.
> So while I like rabbit monoclonals, use them, advocate for them and endorse the
> suggestion you try them, I'm not convinced that as a rule they have 10x higher
> affinity than do mouse monoclonals.
> Ray Koelling
> Phenopath Laboratories
> Seattle, WA
> -------------- Original message ----------------------
> From: "Mildred Fail"
> > We have had quite a problem with CD3s on bone marrow biopsies being
> > "messy" both with mouse monoclonals and rabbit polyclonals. Protein
> > block is used. Diluting the Ab out further lost some cells in the lymph
> > node control. We tried a rabbit monoclonal. The staining is very
> > specific and intense. The slide is beautifully free of extraneous
> > staining. The higher dilution has not appeared to have effected the
> > number of cells stained. Question is why would the rabbit
> > monoclonal produce a cleaner slide?
> > Rena Fail
> > Rena Fail
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