RE: [Histonet] c-fos

From:"Turner, Scott"

Are you sure it's "thermosol" and not "thimerosal"?  Thimerosal is a commonly used preservative and can be found on the Sigma website (CAS Number: 54-64-8).  Thermosol is a process used in commercial dyeing.

Scott Turner
Schering-Plough Biopharma
(formerly DNAX Research Institute)
Palo Alto, CA

-----Original Message-----
From: [] On Behalf Of Delatour Benoît
Sent: Thursday, April 26, 2007 10:51 AM
Subject: [Histonet] c-fos

I also confirm that fos IHC can be performed on frozen sections after 
fixation-cryoprotection without thermosol. If you really need a 
preservative you can use sodium diazide 

Benoît a écrit :
> Message: 8
> Date: Thu, 26 Apr 2007 08:56:36 -0400
> From:
> Subject: [Histonet] c-fos staining
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
> I will be carrying out c-fos straining, and according to the protocol 
> I am following, I need to preserve my tissue in a mixture of succrose 
> and Thermosol. Thermosol is a preservative, however, I can't seem to 
> find it anywhere (i.e sigma, fluka etc.) Is there another 
> chemical/preservative that I can use instead, or even another 
> technique?
> Jaina

Laboratoire de Neurobiologie de l'Apprentissage,
de la Mémoire & de la Communication,
NAMC, CNRS UMR 8620, Bât 446
Université Paris-Sud
91405 Orsay Cedex, FRANCE

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