RE: [Histonet] alcian blue_ precipitation

From:"Tony Henwood"

Alcian blue tetrakis (methylpyridinium) chloride (Sigma, Cat. No. A4045,
90% dye content) is a better dye to use. See  "Improved demonstration of
mast cells using alcian blue tetrakis (methylpyridium) chloride"
Biotechnic & Histochemistry 77(2):93-94.(2002)


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of
Sent: Wednesday, 18 April 2007 12:09 AM
To: raghul;
Subject: re: [Histonet] alcian blue_ precipitation

> Raghul,

try dissolving the Alcian blue powder in distilled water first, then
adjust the pH with acid. You should have no problem with pH 2.5, but
some lot #s of dye will precipitate at pH 1.0. If this happens contact
me again.


> dear histonetters
>   When i try to dissolve the alcian blue (sigma) powder in acidified 
> (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 
> 1:1 alchol:distilled water adjusted to required pH its dissolving but 
> isnt staining any of mucin cells in positive control. please help
>   raghul
>   Medbiotech res. limited
>   chennai, India
> ---------------------------------
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