RE: [Histonet] alcian blue_ precipitation

From:"Tony Henwood"

Dissolve the dye in water first and then add diluted acid in water.
Eg for 1% alcian blue in 2.5% acetic acid:
1g dye in 50ml distilled water.
2.5ml acetic acid in 47.5ml water.
When the dye has dissolved, mix the two solutions


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of raghul
Sent: Tuesday, 17 April 2007 11:22 PM
Subject: [Histonet] alcian blue_ precipitation

dear histonetters
  When i try to dissolve the alcian blue (sigma) powder in acidified
(ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1
alchol:distilled water adjusted to required pH its dissolving but isnt
staining any of mucin cells in positive control. please help
  Medbiotech res. limited
  chennai, India

 Check out what you're missing if you're not on Yahoo! Messenger 
Histonet mailing list

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

Histonet mailing list

<< Previous Message | Next Message >>