We use Clark's fixative = Carnoy's without chloroform. It works well
even after the specimen has been in formalin.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
rsrichmond@aol.com
Sent: Tuesday, April 24, 2007 1:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Finding Lymph Nodes After Fixation
Wanda Smith asks:
>>One of my pathologists asked me to ask a question - Has anyone had
success in finding colonic lymph nodes in fat AFTER fixation with 10%
neutral buffered formalin? This pathologist has tried Dissect-aid and
other solutions that seem to work on fresh fat, but he has had no luck
trying this after the fat has been in formalin.<<
No, this doesn't work. Though often when the specimen arrives in
formalin the fat has been so little penetrated by formalin that using a
disclosing fixative is still worthwhile.
Any other specialty, you'd be able to make arrangements with the
operating room staff to solve this problem. In pathology, the red-haired
stepchild again. And the clinical stakes are high here - one positive
lymph node, however small, requires chemotherapy.
Bob Richmond
Samurai Pathologist
Knoxville TN
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free
from AOL at AOL.com.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|