No you do not have to heat Faramount. Use it straight from the bottle.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Tuesday, April 24, 2007 11:34 AM
To: Martin S.; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Aqueous mountant suggestions please
The Faramount works, but it must be heated before coverglass
application,
and the heating adds another step you have to deal with, Howevere, it is
very nice aqueous media that sets up hard and clear.
We have also done frozen sections with DAB and set up the
dehydration/clearing for permanent mounting media. I must admit this
gives
a superb final preparation that when dry, the mounting media doesn't
retract under the coverglass. It is not that difficult to set up the
dehyration/clearing/permanent mountant, and there are some nice
mini-staining devices to help you out depending on how many slides you
stain at one time. We prefer dehydration/clearing/and permanent media
with DAB.
At 09:39 AM 4/24/2007, you wrote:
>Quick query,
>
>I need and aqueous mountant for immunohistochemistry slides. I am
>currently using Vectamount AQ from Vectashield but am having some
>problems with it not hardening completely and it doesnt seem to last
>very long ie. the use by date is already up and I'm only halfway
through
>the bottle.
>
>Mostly I am mounting immunolabelled frozen tissue sections with DAB. I
>know aqueous mountants are generally used for AEC but I dont see the
>point in doing the alcohol and xylene steps if an aqueous mountant
>works. I dont do paraffin sections so dont already have this set-up.
>
>Vector say that their aqueous mountant is also suitable for DAB but is
>this true for all aqueous mountants? I've been looking at Faramount
>Aqueous mountant from Dako - any comments?
>
>Thanks
>Sonya
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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