RE: [Histonet] Oil Red O Controls

From:"Woodward, Denise"



A hot dog works terrific as an Oil Red O or Sudan Black B control.
'Course, you will never eat another one after you see the "tissue" it contains!
Happy trails,
Denise Long Woodward
University of Connecticut 
Dept. of Pathobiology and Veterinary Sciences
Storrs, CT  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evelyn Kaplan
Sent: Monday, April 16, 2007 11:43 PM
To: 'Rene J Buesa'; 'Greg Dobbin'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Oil Red O Controls

I have used a piece of fixed fish liver for many years (not the same one,
that is! :-)) and it makes an easy source of fat control tissue. 

 

Evelyn Kaplan,

Dept. of Medical Education,

Chair, BSc MLT Programme,

Faculty of Medicine & Health Sciences,

Al Ain, UAE.

 

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 16 April 2007 16:49
To: Greg Dobbin; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil Red O Controls

 

I used to cut FS of (+) materials and stored UNFIXED in a -80║C freezer.
They were useful for several years.

  RenÚ J.

 

Greg Dobbin  wrote:

  Hello All,

I am interested in hearing what other labs are doing with regard to having

good positive controls slides on hand. Are frozen sections cut from normal

breast tissue and stored? How? Fixed or unfixed? Room temp or freezer?

Thanks in advance!

 

-- 

 

Greg Dobbin

 

Chief Technologist Histology

Queen Elizabeth Hospital

Charlottetown, PE

Canada

Ph (902) 894-2337

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