RE: [Histonet] Cell Button Prep

From:"Kemlo Rogerson"

You could try the hypaque method. If you are interested I could get the
method but it bluntly uses a density gradient. You spin down the fluid
remove supernatant and reconstitute with saline. You make a solution of
hypaque and layer the cell/ saline solution on top. You then spin, rbcs
and crude fall through the hypaque and larger cells float on top of the
hypaque. Harvest these cells, wash and then mix with agar and cool.
Process and cut and stain. Job done.

But ThinPrep is the way forward from my experience and if you are clever
you can come up with some sedimentation process that might emulate
ThinPrep similar to the one SurgiPath was flogging; I think it was
French but god knows what happened to it.   

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
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