We recently had this same problem and traced it to the water containers on our automated stainer.  I bleached them out really well, but the bacteria were still there, so now we run all specials for microorganisms down by hand in our special stains area.

Laurie Colbert

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Tracey.Lenek@CLS.ab.ca
Sent: Tuesday, April 24, 2007 1:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB Contamination


Hi,

We are experiencing transient false positive ZN staining on skin biopies.  Today a slide had clumped AFB over the majority of the slide where no tissue was present.  Previous to this, only one or two bacilli were noted on the slide.
Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our  lab reuses both?  Any help would be appreciated.

Tracey


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