RE: [Histonet] AFB Contamination

From:"Liz Chlipala"


There is a ton of mycobacterium in tap water and in the plumbing system.  We
always use filtered water in our water bath for AFB staining.  Check out
Freidas book and staining for AFB.  The culprit is probably your waterbath,
but it could also be your AFB stain.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of
Sent: Tuesday, April 24, 2007 2:14 PM
Subject: [Histonet] AFB Contamination


We are experiencing transient false positive ZN staining on skin biopies.
Today a slide had clumped AFB over the majority of the slide where no tissue
was present.  Previous to this, only one or two bacilli were noted on the
Has anyone investigated and found a contaminated waterbath or perhaps the
Kinyoun's is the culprit as our  lab reuses both?  Any help would be


CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain
confidential, personal and/or privileged information intended for a specific
purpose and recipient. If you are not the intended recipient do not
disclose, copy, retain, distribute, use or modify any of the contents of
this transmission. If you received this transmission in error please notify
me immediately by return e-mail or telephone and destroy the entire
transmission and any copies produced. Thank you.

Histonet mailing list

__________ NOD32 1971 (20070110) Information __________

This message was checked by NOD32 antivirus system.

Histonet mailing list

<< Previous Message | Next Message >>