I also confirm that fos IHC can be performed on frozen sections after 
fixation-cryoprotection without thermosol. If you really need a 
preservative you can use sodium diazide 
(http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA).

Benoît

histonet-request@lists.utsouthwestern.edu a écrit :
> Message: 8
> Date: Thu, 26 Apr 2007 08:56:36 -0400
> From: jaina.negandhi@sickkids.ca
> Subject: [Histonet] c-fos staining
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> 	
> Content-Type: text/plain; charset=US-ASCII
>
> I will be carrying out c-fos straining, and according to the protocol I am
> following, I need to preserve my tissue in a mixture of succrose and
> Thermosol. Thermosol is a preservative, however, I can't seem to find it
> anywhere (i.e sigma, fluka etc.) Is there another chemical/preservative
> that I can use instead, or even another technique?
>
> Jaina
>   

-- 
Laboratoire de Neurobiologie de l'Apprentissage,
de la Mémoire & de la Communication,
NAMC, CNRS UMR 8620, Bât 446
Université Paris-Sud
91405 Orsay Cedex, FRANCE
Email  benoit.delatour@u-psud.fr
Web    http://www.namc.u-psud.fr


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