Well, three different people have claimed they can get away with
post-fixing colonic mesenteries in a disclosing fixative to look for
lymph nodes. I'm surprised - theoretically it shouldn't work, in my
personal experience it doesn't work, and several people have told me
that they couldn't make it work - so I felt justified in pontificating.
Well, I'll try it again if the opportunity arises.
I wonder if the mesenteries received in formalin were adequately fixed
- it's a situation where formalin penetrates very slowly.
My personal preference is Davidson's fixative (3 parts water, 3 parts
alcohol, 2 parts 37% formaldehyde solution, 1 part glacial acetic
acid). Surgipath's O-Fix in my experience works quite as well. I've had
no luck Googling "Presto-Fixe II from spectra-tint" - look at the MSDS
and tell us what's in it.
Once again I want to emphasize the clinical seriousness of this problem
- lymph node metastases from colon cancer are often the size of
pinheads, very hard to find - and their presence determines treatment.
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